bsm-62980R [Primary Antibody]
Phospho-SMC1 alpha (Ser957) Recombinant Antibody
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Host: Rabbit

Target Protein: SMC1 alpha

Modification Site: Ser957

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 8243

Swiss Prot: Q14683

Source: KLH conjugated synthetic peptide derived from human SMC1 alpha around the phosphorylation site of S957

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped.

Size: 100µL

Concentration: Lot dependent

Predicted Molecular Weight: 143


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Western blot analysis of (1) HeLa cell lysate; (2) Hela cells lysate treated with AP. Using Phospho-SMC1 alpha (Ser957) (bsm-62980R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SMC1 alpha (Ser957) Monoclonal Antibody, Unconjugated (bsm-62980R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SMC1 alpha (Ser957) Monoclonal Antibody, Unconjugated (bsm-62980R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SMC1 alpha (Ser957) Monoclonal Antibody, Unconjugated (bsm-62980R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SMC1 alpha (Ser957) Monoclonal Antibody, Unconjugated (bsm-62980R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SMC1 alpha (Ser957) Monoclonal Antibody, Unconjugated (bsm-62980R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SMC1 alpha (Ser957) Monoclonal Antibody, Unconjugated (bsm-62980R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SMC1 alpha (Ser957) Monoclonal Antibody, Unconjugated (bsm-62980R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SMC1 alpha (Ser957) Monoclonal Antibody, Unconjugated (bsm-62980R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SMC1 alpha (Ser957) Monoclonal Antibody, Unconjugated (bsm-62980R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SMC1 alpha (Ser957) Monoclonal Antibody, Unconjugated (bsm-62980R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SMC1 alpha (Ser957) Monoclonal Antibody, Unconjugated (bsm-62980R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SMC1 alpha (Ser957) Monoclonal Antibody, Unconjugated (bsm-62980R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SMC1 alpha (Ser957) Monoclonal Antibody, Unconjugated (bsm-62980R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SMC1 alpha (Ser957) Monoclonal Antibody, Unconjugated (bsm-62980R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SMC1 alpha (Ser957) Monoclonal Antibody, Unconjugated (bsm-62980R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SMC1 alpha (Ser957) Monoclonal Antibody, Unconjugated (bsm-62980R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SMC1 alpha (Ser957) Monoclonal Antibody, Unconjugated (bsm-62980R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.