bsm-63002R [Primary Antibody]
Thioredoxin Recombinant Antibody
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Host: Rabbit

Target Protein: Thioredoxin

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 7295

Swiss Prot: P10599

Source: KLH conjugated synthetic peptide derived from human Thioredoxin

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity.

Size: 100µL

Concentration: Lot dependent

Predicted Molecular Weight: 12


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with Thioredoxin monoclonal antibody, unconjugated (bsm-63002R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.