support@biossusa.com
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
Host: Rabbit
Target Protein: FOXP3
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 50943
Swiss Prot: Q9BZS1
Source: A synthesized peptide derived from human FOXP3
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1mg/ml
Applications:
WB(WB=1:500-2000)
IHC-P(IHC-P=1:50-200)
IHC-F(IHC-F=1:50-200)
IP(IP=1:50-100)
IF(IF=1:50-200)
Predicted Molecular Weight: 47
Cross Reactive Species:
Human
Mouse
For research use only. Not intended for diagnostic or therapeutic use.
25 ug total protein per lane of various lysates (see on figure) probed with FOXP3 monoclonal antibody, unconjugated (bsm-63030R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FOXP3 Monoclonal Antibody, Unconjugated (bsm-63030R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.