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Host: Rabbit
Target Protein: phospho-BAD (S75)
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 572
Swiss Prot: Q92934
Source: KLH conjugated Synthesised phosphopeptide derived from human BAD around the phosphorylation site of Ser75
Purification: Purified by Protein A.
Storage Buffer: 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100uL
Concentration: Lot Dependent
Applications: WB(WB(1:500-2000))
Predicted Molecular Weight: 18
Cross Reactive Species: Human
Predicted Cross Reactive Species:
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
Hela (H) cells were treated with or without Calyculin A (50nM) for 30 min, 25 μg total protein per lane of cell lysates (see on figure) probed with phospho-BAD (S75) monoclonal antibody, unconjugated (bsm-63059R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.