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Host: Rabbit
Target Protein: phospho-Tau (S396)
Modification Site: S396
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 4137
Swiss Prot: P10636
Source: KLH conjugated Synthesised phosphopeptide derived from human Tau around the phosphorylation site of Ser396
Purification: Purified by Protein A.
Storage Buffer: 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100µL
Concentration: Lot Dependent
Predicted Molecular Weight: 79
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-Tau (S396)Monoclonal Antibody, Unconjugated(bsm-63063R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-Tau (S396)Monoclonal Antibody, Unconjugated(bsm-63063R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-Tau (S396)Monoclonal Antibody, Unconjugated(bsm-63063R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
25 ug total protein per lane of various lysates (see on figure) probed with phospho-Tau (S396) monoclonal antibody, unconjugated (bsm-63063R) at 1:4000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.