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Host: Rabbit
Target Protein: 4-Oct
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 5460
Swiss Prot: Q01860
Source: KLH conjugated synthetic peptide derived from human OCT4
Purification: Purified by Protein A.
Storage Buffer: 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100µL
Concentration: 1mg/ml
Applications:
WB(WB(1:500-2000))
IHC-P(FCM(1:50-100))
IHC-F(IHC-P(1:50-200))
IF(IHC-F(1:50-200))
Flow-Cyt(IF(IHC-P)(1:50-200))
Predicted Molecular Weight: 39
Cross Reactive Species: Human
Predicted Cross Reactive Species: Mouse
For research use only. Not intended for diagnostic or therapeutic use.
25 ug total protein per lane of various lysates (see on figure) probed with Oct4 monoclonal antibody, unconjugated (bsm-63076R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.