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Host: Rabbit
Target Protein: Histone H3.3(T3)
Clonality: Recombinant
Isotype: IgG
Swiss Prot: P84243
Source: A synthesized peptide derived from human Histone H3.3 around the phosphorylation site of T3
Purification: Purified by Protein A.
Storage Buffer: 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100µl
Concentration: Lot dependent
Applications:
WB(WB=1:500-2000)
IHC-P(IHC-P=1:100-200)
IHC-F(IHC-F=1:100-200)
IF(IF=1:50-200)
Flow-Cyt(Flow-Cyt=1:50-100)
ICC/IF(ICC/IF=1:100-500)
Predicted Molecular Weight: 15
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
Hela (H) cells were treated with or without Calyculin A (100nM) for 30 min, 25 μg total protein per lane of cell lysates (see on figure) probed with phospho-Histone H3.3(T3) monoclonal antibody, unconjugated (bsm-63098R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Histone H3.3(T3) Monoclonal Antibody, Unconjugated (bsm-63098R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Histone H3.3(T3) Monoclonal Antibody, Unconjugated (bsm-63098R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Histone H3.3(T3) Monoclonal Antibody, Unconjugated (bsm-63098R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Histone H3.3(T3) Monoclonal Antibody, Unconjugated (bsm-63098R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Histone H3.3(T3) Monoclonal Antibody, Unconjugated (bsm-63098R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Glioblastoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Histone H3.3(T3) Monoclonal Antibody, Unconjugated (bsm-63098R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.