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Host: Rabbit
Target Protein: EIF2AK2
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 5610
Swiss Prot: P19525
Source: A synthesized peptide derived from human eIF2A K2
Purification: Purified by Protein A.
Storage Buffer: 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: Lot dependent
Applications:
WB(WB=1:500-2000)
IHC-P(IHC-P=1:100-200)
IHC-F(IHC-F=1:100-200)
IP(IP=1:20-50)
IF(IF=1:50-200)
ICC/IF(ICC/IF=1:50-200)
Predicted Molecular Weight: 62
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
25 ug total protein per lane of various lysates (see on figure) probed with EIF2AK2 monoclonal antibody, unconjugated (bsm-63145R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with EIF2AK2 Monoclonal Antibody, Unconjugated (bsm-63145R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with EIF2AK2 Monoclonal Antibody, Unconjugated (bsm-63145R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with EIF2AK2 Monoclonal Antibody, Unconjugated (bsm-63145R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.