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Host: Rabbit
Target Protein: Histone H3(S28)
Clonality: Recombinant
Isotype: IgG
Swiss Prot: P68431
Source: A synthesized peptide derived from human Histone H3.1 around the phosphorylation site of S28
Purification: Purified by Protein A.
Storage Buffer: 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: Lot dependent
Predicted Molecular Weight: 15
Cross Reactive Species: Human
Predicted Cross Reactive Species:
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
Hela (H) cells were treated with or without calyculin A (100nM) for 30 min, 25 μg total protein per lane of cell lysates (see on figure) probed with phospho-Histone H3(S28) monoclonal antibody, unconjugated (bsm-63239R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.