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Host: Rabbit
Target Protein: PRKAR2A
Clonality: Recombinant
Isotype: IgG
Swiss Prot: P13861
Source: A synthesized peptide derived from human PRKAR2A
Purification: Purified by Protein A.
Storage Buffer: 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100µl
Concentration: Lot dependent
Applications:
WB(WB=1:1000-2000)
IHC-P(IHC-P=1:100-200)
IHC-F(IHC-F=1:100-200)
IP(IP=1:20-50)
IF(IF=1:50-200)
ICC/IF(ICC/IF=1:50-200)
Predicted Molecular Weight: 46
Cross Reactive Species: Human
Predicted Cross Reactive Species: Cow
For research use only. Not intended for diagnostic or therapeutic use.
25 ug total protein per lane of various lysates (see on figure) probed with PRKAR2A monoclonal antibody, unconjugated (bsm-63288R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PRKAR2A Monoclonal Antibody, Unconjugated (bsm-63288R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Adrenal Gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PRKAR2A Monoclonal Antibody, Unconjugated (bsm-63288R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PRKAR2A Monoclonal Antibody, Unconjugated (bsm-63288R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (PRKAR2A) monoclonal Antibody, unconjugated (bsm-63288R) 1:100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37°C for 90 min, then stained with Phalloidin/BF555 for 30 min at room temperature; DAPI (blue, C02-04002) was used to stain the cell nuclei.
4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (PRKAR2A) monoclonal Antibody, unconjugated (bsm-63288R) 1:100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37°C for 90 min, then stained with Phalloidin/BF555 for 30 min at room temperature; DAPI (blue, C02-04002) was used to stain the cell nuclei.