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Host: Rabbit
Target Protein: ERK1/ERK2 (p-T202/Y204/T185/Y187)
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 5594
Swiss Prot: P27361
Source: A synthesized peptide derived from human ERK1 around the phosphorylation site of T202/Y204
Purification: purified by Protein A.
Storage Buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1mg/ml
Applications:
WB(1:500-2000)
Flow-Cyt(1:100-200)
ICC/IF(1:100-200)
Cross Reactive Species:
Human
Rat
Predicted Cross Reactive Species:
Mouse
Pig
For research use only. Not intended for diagnostic or therapeutic use.
25 ug total protein per lane of various lysates (see on figure) probed with phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) monoclonal antibody, unconjugated (bsm-63322R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.