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Host: Rabbit
Target Protein: PKA C-alpha/beta/gamma (p-T197)
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 5566
Swiss Prot: P17612
Purification: purified by Protein A.
Storage Buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: Lot Dependent
Applications:
IHC-P(1:50-200)
IHC-F(1:50-200)
WB(1:500-2000)
IF(1:50-200)
ELISA(1:5000-10000)
IP(1:50-200)
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
Jurkat (H) serum starve for 24 h then 20% FBS 20 h , 25 μg total protein per lane of cell lysates (see on figure) probed with Phospho-PKA C-alpha & beta & gamma (Thr197) monoclonal antibody, unconjugated (bsm-63522R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with Phospho-PKA C-alpha & beta & gamma (Thr197) monoclonal antibody, unconjugated (bsm-63522R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKA C-alpha&beta&gamma (p-T197) Monoclonal Antibody, Unconjugated (bsm-63522R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKA C-alpha&beta&gamma (p-T197) Monoclonal Antibody, Unconjugated (bsm-63522R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKA C-alpha&beta&gamma (p-T197) Monoclonal Antibody, Unconjugated (bsm-63522R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKA C-alpha&beta&gamma (p-T197) Monoclonal Antibody, Unconjugated (bsm-63522R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKA C-alpha&beta&gamma (p-T197) Monoclonal Antibody, Unconjugated (bsm-63522R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKA C-alpha&beta&gamma (p-T197) Monoclonal Antibody, Unconjugated (bsm-63522R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKA C-alpha&beta&gamma (p-T197) Monoclonal Antibody, Unconjugated (bsm-63522R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.