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Host: Rabbit
Target Protein: PLK1
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 5347
Swiss Prot: P53350
Purification: purified by Protein A.
Storage Buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul, 20uL
Concentration: Lot Dependent
Applications:
WB(WB=1:500-2000)
ELISA(ELISA=1:5000-20000)
IHC-F(IHC-F=1:200-1000)
IP(IP=1:50-200)
IF(IF=1:200-1000)
IHC-P(IHC-P=1:200-1000)
Predicted Molecular Weight:
Cross Reactive Species:
Human
Mouse
Predicted Cross Reactive Species: Rat
For research use only. Not intended for diagnostic or therapeutic use.
25 ug total protein per lane of various lysates (see on figure) probed with PLK1 monoclonal antibody, unconjugated (bsm-63624R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PLK1 Monoclonal Antibody, Unconjugated (bsm-63624R) at 1:200 overnight at 4°C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PLK1 Monoclonal Antibody, Unconjugated (bsm-63624R) at 1:200 overnight at 4°C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PLK1 Monoclonal Antibody, Unconjugated (bsm-63624R) at 1:200 overnight at 4°C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.