bsm-70008M

DATASHEET

Host: Mouse

Target Protein: Alpha Internexin/ NF-66

Specificity: Specific for endogenous levels of the ~66 kDa alpha Internexin protein. Can be used on formalin-fixed cells in tissue culture, cryostat sections, and Western blotting. The epitope recognized by the ID2 clone is in the C-terminal non-helical extension of the protein and is unusually resistant to aldehyde fixation, so that this antibody is ideal for studies of paraffin embedded formalin fixed histological sections.

Clonality: Monoclonal

Isotype: IgG1

Entrez Gene: 24503

Swiss Prot: P23565

Source: Recombinant rat alpha-internexin expressed in and purified from E. coli

Purification: Purified by Protein G.

Storage Buffer: PBS + 10 mM NaN3.

Storage: Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cy

Background:

Alpha-internexin is a Class IV intermediate filament originally discovered as it co-purifies with other neurofilament subunits (1). Alpha-internexin is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. It is expressed only in neurons and in large amounts early in neuronal development, but is down-regulated in many neurons as development proceeds. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons alphainternexin is the only neurofilament subunit expressed. Antibodies to alpha-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition, recent studies show a marked up-regulation of alpha-internexin during neuronal regeneration (2). The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest. Recently Cairns et al. used this antibody to show that alphainternexin is an abundant component of the inclusions of neurofilament inclusion body disease (NFID), a serious human neurodegenerative disorder (3,4). The antibody was also used to confirm the presence of circulating auto-antibodies to alpha-internexin in the sera of some patients with endocrine autoimmunity, as well as in some normal individuals (5).