bsm-70034M

DATASHEET

Host: Mouse

Target Protein: CD75

Specificity: Specific for endogenous levels of the ~35 kDa CD75 protein.

Clonality: Monoclonal

Isotype: IgM

Entrez Gene: 6480

Swiss Prot: P15907

Source: Nuclei isolated from pokeweed mitogen-stimulated human peripheral blood lymphocytes.

Purification: Protein L purified

Storage Buffer: PBS

Storage: Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cy

Background:

CD75, also known as β-galactoside α2,6-sialyltransferase, is a specific carbohydrate antigen that is predominantly expressed on B cell lymphocytes. β-galactoside α2,6-sialyltransferase is an enzyme that catalyzes the addition of sialic acid to growing carbohydrate chains of glycoproteins (Guy, K., et al, 1991). CD75 is a major component of the lymphoid cell surface that serves as a ligand for cell adhesion molecules (Paulson, J.C., et al, 1989). The LN-1 clone reacts positively with red blood cell precursors of the bone marrow, ciliated epithelial cells of the bronchus, distal tubular cells of the kidney, and ductal cells from several organs including the breast and prostate (Epstein, A.L., et al, 1984). CD75 has been shown to be superior to other B cell markers in detecting lymphocyte predominant cells through their cytoplasmic and membraneous staining, especially in typical nodular lymphocyte-predominant Hodgkin lymphoma patterns (Carbone, A., et al, 2014).

Size: 100µL

Concentration: Lot Dependent

Applications: WB(1:300-5000)
IHC

Predicted Molecular Weight: 35

Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.