DATASHEET
Host:
Mouse
Target Protein:
Fibrillarin
Specificity:
Specific for endogenous levels of the ~34 kDa Fibrillarin /Nop1p protein.
Clonality:
Monoclonal
Isotype:
IgG1
Entrez Gene:
851548
Swiss Prot:
P15646
Source:
Yeast nuclear preparation
Purification:
Purified by Protein G.
Storage Buffer:
PBS + 50% glycerol and 5 mm NaN3
Storage:
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Background:
Nop1p was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae. The Nop1p protein is 327 amino acids in size (34.5kDa), is essential for yeast viability, and is localized in the nucleoli (1). The systematic name for S. cerevisiae Nop1 is YDL014W, and it is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of a protein found in all eukaryotes and archaea generally called fibrillarin (2). Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67% identical, and the human protein can functionally replace the yeast protein. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a ~34 kDa protein which was subsequently found to be fibrillarin. Recent studies show that knock-out of the fibrillarin gene in mice results in embryonic lethality, although mice with only one functional fibrillarin/Nop1p gene were viable (3). This antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species (e.g. 4-6).
Size:
100ul
Concentration:
Lot Dependent
34
Human
Mouse
Rat
Bovine
Pig
Chicken
Drosophila
Predicted Cross Reactive Species:
Dog
Sheep
Horse
Cat
Rabbit
Guinea Pig
Goat
Hamster
Non-Human Primate
Vole
For research use only. Not intended for diagnostic or therapeutic use.