DATASHEET
Host:
Mouse
Target Protein:
Microtubule Associated Protein 2 (MAP2)
Specificity:
Specific for endogenous levels of the ~280 kDa MAP2 protein.
Clonality:
Monoclonal
Isotype:
IgG1
Entrez Gene:
4133
Swiss Prot:
P11137
Source:
Purified bovine MAP2.
Purification:
Purified by Protein G.
Storage Buffer:
PBS + 50% glycerol and 5 mM NaN3
Storage:
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Background:
Microtubules are 25nm diameter protein rods found in most kinds of eukaryotic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein ? (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5 (Kindler & Gardner 1994). MAP2 is made up of two ~280 kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70 kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein tau. MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. MAP2 has been recently shown to be the specific receptor for the neurosteroid pregnenolone (Fontaine-Lenore V. et al., 2006).