bsm-70173M

DATASHEET

Host: Mouse

Target Protein: Neurofilament NF-M

Specificity: Specific for endogenous levels of the ~145 kDa neurofilament M protein.

Clonality: Monoclonal

Isotype: IgG1

Entrez Gene: 24588

Swiss Prot: P12839

Source: Preparation containing the extreme C-terminus of rat NF-M, expressed in and purified from E. Coli.

Purification: Purified by Protein G.

Storage Buffer: PBS + 10 mM NaN3.

Storage: Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cy

Background:

Neurofilaments are the 10 nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (1). NF-M is the neurofilament middle or medium molecular weight polypeptide and runs on SDS-PAGE gels at 145-160 kDa, with some variability across species boundaries. Antibodies to NF-M are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-M antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) and Alzheimer's disease (2).

Size: 100µL

Concentration: 2 mg/ml

Applications: WB(1:300-5000)
IHC

Predicted Molecular Weight: 145

Cross Reactive Species: Human
Mouse
Rat
Bovine
Chicken

For research use only. Not intended for diagnostic or therapeutic use.