bsm-70306M

DATASHEET

Host: Mouse

Target Protein: Ubiquitin C-terminal Hydrolase 1 (UCHL1)

Specificity: Specific for endogenous levels of the ~24 kDa UCHL1 protein.

Clonality: Monoclonal

Isotype: IgG1

Entrez Gene: 7365

Swiss Prot: P09936

Source: Recombinant full length human UCHL1 purified from E. coli.

Purification: Purified by Protein G.

Storage Buffer: PBS + 50% glycerol and 5 mm NaN3

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Ubiquitin C-terminal hydrolase 1 (UCHL1) is also known as ubiquitin carboxyl esterase L1, ubiquitin thiolesterase, neuron-specific protein PGP9.5 and Park5. It was originally identified as a major component of the neuronal cytoplasm from 2-dimensional gel analysis of brain tissues, and was given the name PGP9.5 (1). It was later found that ubiquitin C-terminal hydrolase enzyme activity was associated with the PGP9.5 protein (2). The ubiquitin C-terminal hydrolases cleave ubiquitin from other molecules. Regulation of the ubiquitin pathway is very important and many disease states are associated with defects in this pathway. Genetic knockout of UCHL1 in mice results in a motor neuron degeneration similar to the spontaneous gracile axonal dystrophy (gad) mutant mice (3). Point mutations in the UCHL1 gene are associated with some forms of human Parkinson's disease (4). Since UCHL1 is heavily expressed in neurons, it is released in large amounts following injury or degeneration, so the detection of UCHL1 in CSF and other bodily fluids can be used as a biomarker.

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 24

Cross Reactive Species: Human
Mouse
Rat
Bovine
Pig

Predicted Cross Reactive Species: Dog
Sheep
Horse
Cat
Rabbit
Guinea Pig
Goat
Hamster
Non-Human Primate
Vole

For research use only. Not intended for diagnostic or therapeutic use.