bsm-70392M

DATASHEET

Host: Mouse

Target Protein: Caveolin-1 (Tyr-14)

Specificity: This antibody detects a 21 kDa* protein corresponding to the apparent molecular mass of caveolin-1 on SDS-PAGE immunoblots of human A431 cells treated with EGF or pervanadate, and is not observed in untreated cells. This sequence has significant homology to the conserved site in rat and mouse Caveolin-1.

Clonality: Monoclonal

Isotype: IgG1

Swiss Prot: Q03135

Source: Clone M283 was generated from a phospho-Caveolin-1 (Tyr-14) synthetic peptide corresponding to amino acids surrounding tyrosine 14 in human Caveolin-1.

Purification: Purified by Protein A.

Storage Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Caveolins are the primary structural components of the plasma membrane microdomains, caveolae. Three members of the caveolin family (caveolin-1, -2, and -3) have been identified, and each has distinct expression patterns. Caveolins are involved in diverse biological functions, including vesicular trafficking, cholesterol homeostasis, cell adhesion and apoptosis. Caveolins can interact with various signaling molecules, including G-proteins, receptor tyrosine kinases, PKCs, and Src family kinases. Phosphorylation at Tyr-14 is essential for caveolin association with SH2 or PTB domain-containing adaptor proteins, while phosphorylation at Ser-80 regulates caveolin binding to the ER membrane and entry into the secretory pathway.