Host: Mouse
Target Protein: Estrogen Receptor α (Tyr-537)
Specificity: This antibody detects several forms of ERα ranging from 66 to 35 kDa* on SDS-PAGE immunoblots of MCF-7 cells treated with pervanadate, and this reactivity is removed after alkaline phosphatase treatment. This sequence is well conserved in rat and mouse ERα, and is also well conserved in ERβ (Tyr-488).
Modification Site: Tyr-537
Clonality: Monoclonal
Isotype: IgG1
Swiss Prot: P03372
Source: Clone M545 was generated from a phospho-ERα (Tyr-537) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding Tyr-537 in human ERα.
Purification: Antigen Affinity purification
Storage Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Background:
Size: 100ul
Predicted Molecular Weight: 35-66
Cross Reactive Species:
Human
Mouse
Rat
Chicken
Xenopus
For research use only. Not intended for diagnostic or therapeutic use.