bsm-70511M

DATASHEET

Host: Mouse

Target Protein: GSK-3β (N-terminal region)

Specificity: The antibody detects a 46 kDa* protein corresponding to the apparent molecular mass of GSK-3β on SDS-PAGE immunoblots of rabbit fibroblasts, mouse brain, as well as human SKN-SH and A431 cells. This sequence is highly conserved in rat and mouse GSK-3β, and has 65% homology to similar regions in GSK-3α.

Clonality: Monoclonal

Isotype: IgG1

Swiss Prot: P49841

Source: Clone M131 was generated from a recombinant protein containing amino acid residues in the N-terminal region of human GSK-3β.

Purification: Purified by Protein A.

Storage Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Glycogen synthase kinase-3 (GSK-3) has been implicated in fundamental cell processes such as cell fate determination, metabolism, transcriptional control, and oncogenesis. Two GSK-3 genes (α and β) have been cloned in mammals and these kinase homologues show strong sequence conservation within their catalytic domain. GSK-3β plays a critical role in cell survival by phosphorylating nuclear factor-κB (NF-κB) p65 subunit, leading to NF-κB transactivation in hepatocytes. Phosphorylation regulates the activity of both GSK-3 genes. MEK1/2 can phosphorylate tyrosine 216 (tyrosine 279 in GSK-3α), which stimulates GSK-3 kinase activity. Tyr-216 phosphorylation is required for GSK-mediated down-regulation of β-catenin activity. Also, TRAIL stimulation can increase Tyr-216 phosphorylation, and GSK-3β activity may suppress TRAIL-induced apoptosis. Inactiviation of GSK-3 occurs through Akt phosphorylation of serine 9 of GSK-3β (Serine 21 in GSK-3α). This phosphorylation may be involved in later phases of neuronal apoptosis.