bsm-70546M

DATASHEET

Host: Mouse

Target Protein: Mitofilin

Specificity: Clone M027 detects a 90 kDa* band corresponding to the molecular mass of mitofilin on SDS-PAGE immunoblots of native or denatured human PC3, MDA-MB-231, A431, A549, and MeWo cell lysates, as well as human breast, skin, and brain tissues.

Clonality: Monoclonal

Isotype: IgG1

Swiss Prot: Q16891

Source: Clone M027 was generated from a proprietary antigen related to human mitofilin expressed in the A431 epidermoid carcinoma cell line.

Purification: Purified by Protein G.

Storage Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Mitofilin is an important organizer of mitochondrial architecture. The mitofilin sequence encodes a polypeptide with a central alpha-helical region with predicted coiled coil domains flanked by globular amino and carboxy termini. There are four isoforms of mitofilin, and 90/91 kDa mitofilin forms have been observed in western blots. Mitofilin is located in the inner mitochondrial membrane and interacts with several protein complexes of the outer membrane, thereby generating contact sites between the two membrane systems of mitochondria. These mitofilin-containing hetero-oligomeric protein complexes form the mitochondrial inner membrane organizing system (MINOS). MINOS integrity is required for the structural maintenance of the inner mitochondrial membrane. This mitochondrial region contains cristae membranes that form large tubular invaginations that protrude into the mitochondrial matrix. These cristae membranes contain the enzyme complexes of the oxidative phosphorylation machinery. MINOS deficiency causes loss of crista junction structures and the detachment of cristae from the inner boundary membrane.