bsm-70562M

DATASHEET

Host: Mouse

Target Protein: Neuropilin-1 (a1 CUB Domain)

Specificity: The antibody detects a 130 kDa* protein corresponding to the apparent molecular mass of neuropilin-1 on SDS-PAGE immunoblots of human recombinant neuropilin-1 and in human PC3 cells. This sequence is highly conserved in rat and mouse neuropilin-1, and has low homology to neuropilin-2.

Clonality: Monoclonal

Isotype: IgG1

Swiss Prot: O14786

Source: Clone M534 was generated from a neuropilin-1 synthetic peptide (coupled to carrier protein) corresponding to amino acids within the a1 CUB domain of human neuropilin-1.

Purification: Purified by Protein G.

Storage Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Neuropilins are transmembrane proteins that contain two CUB domains (a1 and a2), two coagulation factor-like domains (b1 and b2), and a MAM domain in the extracellular region. These proteins have short cytoplasmic domains that include a PDZ-binding motif. The neuropilin (NRP) family includes NRP-1, NRP-2A, and NRP-2B. NRP-1 has been implicated as a receptor involved in axon guidance and VEGF signaling. NRP-1 mediates activation of intracellular signaling pathways through interaction with its co-receptors, Plexin-A1 and VEGFRs. The expression of NRP-1, along with the co-receptor Plexin-A1, is upregulated in neurons after central nervous system injury. The axons from these neurons cannot cross semaphorin 3A-containing regions at the site of injury. Thus, semaphorin 3A and its co-receptors, Plexin-A1 and Neuropilin-1, may have significant roles in axon regeneration after neuronal injury.