bsm-70605M

DATASHEET

Host: Mouse

Target Protein: Anti-Phosphoserine/threonine

Specificity: The antibody detects many serine or threonine phosphorylated proteins by western blot, immunocytochemistry, and ELISA.

Modification Site: pSer/Thr

Clonality: Monoclonal

Isotype: IgG1

Swiss Prot: N/A

Source: Phosphoserine/threonine is a mix of Clone psA and clone psB. They were generated from synthetic peptides of phosphoserine and phosphothreonine (coupled to carrier protein), respectively.

Purification: Purified by Protein A.

Storage Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Phosphorylation of specific serine or threonine residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor ser/thr kinases that mediate these protein modifications. Antibodies that can detect phosphoserine or phosphothreonine residues are excellent tools for characterizing changes in the post-translational state of a broad range of phosphorylated proteins. Immunoprecipitation of proteins of interest followed by detection of phosphoserine or phosphothreonine using anti-phosphoserine antibody is commonly used to correlate changes in phosphorylation state with alterations in protein activity.

Size: 100µL

Applications: WB(1:200-500)
IP(1:50)

Predicted Molecular Weight: N/A

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.