bsm-70615M

DATASHEET

Host: Mouse

Target Protein: PKCθ (N-terminal region)

Specificity: This antibody detects a 79 kDa* protein corresponding to the molecular mass of PKCθ on SDS-PAGE immunoblots of human Jurkat cell lysates. This sequence is conserved in human and rat PKCθ, and has low homology to other PKC family members.

Clonality: Monoclonal

Isotype: IgG2a

Swiss Prot: A6H667

Source: Clone (M217) was generated from a recombinant mouse PKCθ protein that included amino acids residues in the N-terminal region.

Purification: Purified by Protein A.

Storage Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half interacts with PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester, while the C-terminal half contains the catalytic domain. The conventional PKC subfamily (α, β1, βII, and γ) is regulated by both Ca2+ and diacylglycerol. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters, and growth factors. The phosphorylation of multiple sites in PKCs regulates their activity