bsm-70616M

DATASHEET

Host: Mouse

Target Protein: PLCγ1 (N-terminal region)

Specificity: The antibody detects a 150 kDa* protein in human A431, Hct116, and Jurkat cells, as well as in mouse brain. This sequence is highly conserved in rat and mouse PLCγ1, and has low homology to PLCγ2.

Clonality: Monoclonal

Isotype: IgG1

Swiss Prot: P19174

Source: Clone (M156) was generated from a synthetic peptide corresponding to amino acids in the N-terminal region of human PLCγ1.

Purification: Purified by Protein A.

Storage Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli such as hormones, growth factors, and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate two secondary messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). At least four families of PLCs have been identified: PLCβ, PLCγ, PLCδ, and PLCε. Phosphorylation is one of the key mechanisms that regulates the activity of PLC. PLCδ is activated by both receptor and nonreceptor tyrosine kinases. PLCγ1 forms a complex with EGF and PDGF receptors, which leads to phosphorylation at tyrosine 771, 783, and 1245. In addition, antigen receptor-induced activation of PLCγ1 leads to phosphorylation at both Tyr-775 and Tyr-783. These two sites are equally important for activation of enzymatic activity.

Size: 100uL

Applications: WB(1:300-5000)
IP(1-2ug)

Predicted Molecular Weight: 150

Cross Reactive Species: Human
Mouse
Rat
Chicken

For research use only. Not intended for diagnostic or therapeutic use.