bsm-70686M

DATASHEET

Host: Mouse

Target Protein: Stat3 (N-terminal region)

Specificity: The antibody detects 86 and 79 kDa* bands corresponding to Stat3 isoforms on SDS-PAGE immunoblots of human A431 cells. This sequence has high homology to the conserved regions in rat and mouse Stat3 α and β.

Clonality: Monoclonal

Isotype: IgG1

Swiss Prot: P40763

Source: Clone (M263) was generated from a recombinant protein that included amino acid residues in the N-terminal region of human Stat3.

Purification: Purified by Protein A.

Storage Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

The stat proteins (Stat1-6) function both as cytoplasmic signal transducers and as activators of transcription in response to cytokines and growth factor receptors. Stat3 is expressed as two variants, Stat3α (86 kDa) and Stat3b (79 kDa) that can differ in expression and activity depending on cell type, activation pathway, and cell maturation stage. Both are activated by phosphorylation at Tyr-705, which induces dimerization, nuclear translocation and DNA binding. Stat3α (86 kDa) transcriptional activation may be regulated by phosphorylation at Ser-727 through the MAPK pathway, while Stat3β lacks this serine site.

Size: 100uL

Applications: WB(1:300-5000)
IP(1-2ug)

Predicted Molecular Weight: 79/86

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.