support@biossusa.com
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
Host: Rabbit
Target Protein: Phosphatidylethanolamine Binding Protein 1
Specificity: Knockdown Validated
Immunogen Range: 64-99/187
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 5037
Swiss Prot: P30086
Source: A synthesized peptide derived from human PEBP1
Purification: Affinity Purified
Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage: -20 °C
Background:
Size: 50µL Ab + 25µg cell Lysate + 25µg WT cell, 50µL
Concentration: Lot dependent
Applications:
WB(1:300-5000)
FCM(1:20-100)
IC(1:1000)
Predicted Molecular Weight: 21 kDa
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
Flow cytometric analysis of PEBP1 expression in HeLa cells using PEBP1 antibody (1:2,000). Green, isotype control; red, PEBP1.
Immunocytochemical staining of Hela cells with anti-PEBP1 antibody (1:1,000). Nuclei were stained blue with DAPI; PEBP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.
Western blotting analysis using anti-PEBP1 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PEBP1 antibody (1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit.
Western blotting analysis using anti-PEBP1 antibody. PEBP1 expression in wild type (WT) and PEBP1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates . β-Tubulin serves as a loading control. The blot was incubated with anti-PEBP1 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit.